2130. Neonatal Immune Phenotypes and Neurodevelopmental Outcomes in Infants with Congenital Cytomegalovirus Infection (cCMV)

Abstract Background Infants with congenital cytomegalovirus infection (cCMV) are at risk for neurodevelopmental impairment (NDI). Antiviral therapy has been shown to improve outcomes but is not recommended all infected infants. There currently no predictive markers NDI in infants cCMV. Our objective was compare neonatal T cell differentiation and memory phenotypes CMV-infected without subsequent NDI. Methods Blood samples were collected from cCMV age-matched healthy controls. Peripheral blood mononuclear cells analyzed by flow cytometry CD4 CD8 expressing CD28, CD57, PD, CD45RA/RO, CCR7. Demographic clinical data collected. defined as Bayley III/IV testing below the average range least one domain whom this developmental available or based on diagnosis infectious disease physician primary care provider. Outcomes follow-up 6 months of age included. isolated speech delay associated hearing loss considered have Results Samples 18 (Table 1) time (≤ 60 days age) 5 uninfected The frequencies CD8+ CD28low (differentiation), CD57+ (terminal differentiation/senescence), PD1hi (inhibitory exhausted phenotype) CD45RO± (memory) higher group compared that (Figure 1). had lower proportions naïve (CCR7+/CD45RA+) effector memory, TEM (CCR7-/CD45RA-), RA, TEMRA (CCR7-/CD45RA+) than those 2). difference distribution surface marker expression CD4+ cells. Table 1 characteristics (NI) Continuous variables using Mann Whitney U test categorical Fischer’s Exact test. significant differences between NI. Figure 1.CD8+ groups Neurodevelopmental Impairment (NDI) Frequencies lacking CD28 (A), CD57 (B), PD-1 (C), CD45RO (D) ≤ days. CMV normal outcome blue, red, controls black. Data medians [interquartile range]. Groups Test. significantly CD28- (p = 0.002), 0.001), PD-1+ 0.01) CD45RO+ * p 0.05; ** 0.01. 2.T subset distributions among Proportions (A) (B) (CCR7+/CD45RA+), central TCM (CCR7+/CD45RA-), (CCR7-, CD45RA-), red while black gray. Comparisons made NI fewer 0.0001), greater 0.005) different. Conclusion In small cohort CMV, these suggest a more differentiated phenotype period may correlate outcomes. Disclosures Alexandra K. Medoro, MD, Merck: Grant/Research Support.